An autoclave is a device used to sterilize equipment and supplies by subjecting them to high-pressure saturated steam at a temperature of around 121-134 degrees Celsius (250-273 degrees Fahrenheit) for a specific period, typically 15-20 minutes depending on the size of the load and contents. Autoclaves are commonly used in medical, laboratory, and industrial settings to ensure that instruments, media, and other materials are free of potentially harmful microorganisms. The process of sterilization in an autoclave is based on the principle that steam under pressure can achieve temperatures higher than boiling water, effectively killing bacteria, viruses, fungi, and spores.